Once upon a time in the far away kingdom of Dor lived a brave and virtuous mouse with comically oversized ears who dreamt of becoming a knight. Banished from his home for having such lofty ambitions, Despereaux sets off on an amazing adventure with his good-hearted rat friend Roscuro. The two go on a very noble quest to rescue an endangered princess and save an entire kingdom from darkness.

When renowned paleontologist Dr. Grant agrees to accompany a wealthy adventurer and his wife on an aerial tour of Isla Sorna, InGen's former breeding ground for prehistoric creatures, he has no idea that he was brought along for a totally different reason. When their plane crashes and they become stranded, they discover the island's native inhabitants are smarter, faster, fiercer and more brutal than imagined.